| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MYLK3 recombinant protein (Position: E34-K812) was used as the immunogen for the MYLK3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MYLK3 Antibody / Myosin light chain kinase 3 is a anti-MYLK3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: MYLK3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
MYLK3 expression is restricted mainly to cardiac muscle tissue, distinguishing it from other members of the myosin light chain kinase family such as MYLK and MYLK2, which function in smooth and skeletal muscle, respectively. Located on chromosome 16q23.1, the MYLK3 gene is activated during cardiac differentiation and is transcriptionally regulated by cardiac transcription factors including NKX2-5 and GATA4. Its kinase domain shares structural homology with other Ca2+/calmodulin-dependent kinases but exhibits distinct substrate specificity suited to cardiac myosin regulation.
Functional studies demonstrate that MYLK3 enhances cardiac contractility by maintaining phosphorylation of ventricular myosin light chain 2 (MLC2v). Loss-of-function mutations or reduced expression of MYLK3 have been linked to dilated cardiomyopathy and impaired sarcomere organization. Overexpression of MYLK3 increases calcium sensitivity and promotes sarcomere maturation during cardiomyocyte development, emphasizing its importance in cardiac physiology. In experimental models, altered MYLK3 signaling can influence cardiac hypertrophy, mechanical stress responses, and recovery following myocardial injury.
Immunostaining using MYLK3 antibody reveals strong cytoplasmic localization in cardiac muscle fibers, particularly in ventricular tissue. The antibody is widely applied to research focused on cardiac muscle contractility, signal transduction, and kinase-mediated phosphorylation events. MYLK3 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
