| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human MYO1B was used as the immunogen for the MYO1B antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MYO1B Antibody / Myosin IB is a anti-MYO1B Rabbit antibody Recombinant Rabbit Monoclonal clone 30M16 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: MYO1B
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30M16, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC
Biological background
MYO1B antibody is widely used in studies of cell motility, intracellular trafficking, and actin cytoskeleton dynamics. The protein is expressed in a variety of tissues, with high levels found in brain, kidney, and epithelial cells. MYO1B regulates actin dependent processes including lamellipodia extension and endocytic vesicle transport. By detecting MYO1B, researchers can explore how actin motor proteins coordinate signaling and transport at the plasma membrane.
The antibody is suitable for western blotting, immunohistochemistry, immunofluorescence, and flow cytometry. In western blot assays, MYO1B antibody identifies protein bands of expected molecular weight, enabling quantitative assessment of expression. Immunohistochemistry reveals strong staining at actin rich regions of cells and tissues. Immunofluorescence studies highlight dynamic localization of MYO1B in lamellipodia, endocytic vesicles, and actin filament networks.
MYO1B has been implicated in neuronal development, where it contributes to neurite outgrowth and synaptic remodeling. It also plays roles in epithelial polarity and vesicle trafficking, influencing processes such as nutrient uptake and receptor internalization. Dysregulation of MYO1B has been linked to cancer progression, where altered actin dynamics promote invasion and metastasis. By using MYO1B antibody, scientists can evaluate how myosin IB expression changes in development and disease.
In kidney physiology, MYO1B regulates endocytic pathways that control protein reabsorption. Defects in this process may contribute to renal disorders. In cancer research, increased MYO1B activity has been associated with enhanced migratory capacity of tumor cells. Detecting MYO1B with specific antibodies provides insight into how actin motor proteins contribute to both physiological and pathological outcomes.
MYO1B antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
