| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MYOD1 recombinant protein (Position: M1-R120) was used as the immunogen for the MYOD1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MYOD1 Antibody / Myoblast determination protein 1 is a anti-MYOD1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: MYOD1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Functionally, MYOD1 antibody identifies a 320-amino-acid nuclear protein that dimerizes with E proteins to regulate the transcription of myogenic regulatory genes such as MYOG and MYF5. MYOD1 acts as a pioneer factor, remodeling chromatin to establish muscle-specific transcriptional programs. It is indispensable for myogenic lineage commitment and muscle regeneration following injury.
The MYOD1 gene is located on chromosome 11p15.1 and is expressed in skeletal muscle progenitor cells and differentiating myoblasts. MYOD1 operates in coordination with MEF2 family transcription factors to control muscle fiber formation and repair. During development, its expression defines the onset of the myogenic program.
Pathologically, MYOD1 dysregulation is associated with muscle developmental disorders and certain sarcomas. Mutations in MYOD1 have been identified in rhabdomyosarcoma, where aberrant transcriptional activation promotes tumorigenesis. Research using MYOD1 antibody supports studies in muscle development, differentiation, and cancer biology.
MYOD1 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect myogenic transcription factors.
Structurally, Myoblast determination protein 1 contains a bHLH domain that mediates DNA binding and dimerization. Its N-terminal activation domain recruits chromatin remodeling complexes to activate transcription. This antibody facilitates detailed examination of MYOD1's regulatory network in muscle cell fate determination.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
