| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MYORG recombinant protein (Position: Q207-R611) was used as the immunogen for the MYORG antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MYORG Antibody / Myogenesis-regulating glycosidase is a anti-MYORG Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: MYORG
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, MYORG antibody identifies a 714-amino-acid glycosidase belonging to the glycosyl hydrolase family 31. MYORG hydrolyzes terminal sugar residues from oligosaccharides and glycoproteins, participating in lysosomal degradation and protein quality control. It is mainly localized to the endoplasmic reticulum and perinuclear regions, suggesting involvement in post-translational processing rather than extracellular digestion.
The MYORG gene is located on chromosome 9p13.1 and is highly expressed in brain and skeletal muscle. In the central nervous system, MYORG supports astrocyte differentiation and intercellular communication. It is co-regulated with genes controlling lysosomal activity, endoplasmic reticulum homeostasis, and metabolic signaling.
Pathologically, mutations in MYORG cause primary familial brain calcification (PFBC), a neurodegenerative disorder characterized by calcium deposits in basal ganglia and cerebellum. Deficiency in MYORG leads to altered glycosylation and impaired protein turnover in astrocytes. Research using MYORG antibody assists studies of glycoprotein metabolism, lysosomal function, and neurological disease mechanisms.
MYORG antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect intracellular glycosidases and neuronal processing enzymes.
Structurally, Myogenesis-regulating glycosidase contains a catalytic domain typical of glycosyl hydrolases with conserved acid-base residues, and a C-terminal transmembrane region anchoring it to intracellular membranes. This antibody supports analysis of MYORG's biochemical function and contribution to neurodegenerative disorders.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
