| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MYOT recombinant protein (Position: H9-E494) was used as the immunogen for the MYOT antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MYOT Antibody / Myotilin is a anti-MYOT Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, cell membrane.
Key elements and design rationale
- Target: MYOT
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, FACS, ELISA
Biological background
Structurally, Myotilin is a 57 kilodalton cytoplasmic protein containing two immunoglobulin-like domains at its C-terminus that mediate interactions with alpha-actinin and filamin. Its N-terminal serine-rich region supports actin binding and cross-linking, anchoring thin filaments at the Z-disc. Myotilin is expressed predominantly in skeletal and cardiac muscle, where it acts as a scaffold linking actin filaments to structural and signaling molecules that maintain contractile function.
The MYOT antibody is widely used in muscle biology, myopathy, and cytoskeletal research to study sarcomere structure, actin filament organization, and myofibrillogenesis. Western blot analysis identifies a 57 kilodalton band corresponding to Myotilin, while immunohistochemistry shows distinct Z-disc staining in muscle fibers. This antibody supports investigations into muscle development, maintenance, and degenerative conditions affecting the sarcomere.
Mutations in MYOT cause various myofibrillar myopathies, including limb-girdle muscular dystrophy type 1A and spheroid body myopathy. These mutations lead to Myotilin aggregation, sarcomeric disorganization, and progressive muscle weakness. Altered MYOT expression has also been linked to cardiac remodeling and hypertrophy. The MYOT antibody provides a critical tool for examining these pathogenic mechanisms and evaluating Myotilin's interactions with actin-binding and signaling proteins.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
