| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NAA60 recombinant protein (Position: M1-M242) was used as the immunogen for the NAA60 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NAA60 Antibody / N-alpha-acetyltransferase 60 is a anti-NAA60 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Golgi apparatus.
Key elements and design rationale
- Target: NAA60
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, IP, ELISA
Biological background
Functionally, NAA60 antibody identifies a 242-amino-acid acetyltransferase localized to the cytosolic face of the Golgi apparatus. NAA60 catalyzes the transfer of an acetyl group from acetyl-CoA to protein N-termini, particularly targeting lysine residues within membrane-bound substrates. Its unique subcellular localization distinguishes it from other N-terminal acetyltransferases (NATs) acting in the cytosol or nucleus.
The NAA60 gene is located on chromosome 16p13.3 and is broadly expressed in mammalian tissues, with enrichment in epithelial and secretory cells. By modifying transmembrane proteins, NAA60 influences vesicle trafficking, protein sorting, and Golgi membrane organization. It also contributes to cell cycle progression and mitotic spindle stability.
Pathologically, altered NAA60 activity has been linked to cell proliferation abnormalities and tumorigenesis. Reduced NAA60 expression affects Golgi morphology and disrupts intracellular transport. Research using NAA60 antibody supports studies in protein acetylation, Golgi biology, and membrane-associated protein regulation.
NAA60 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect acetyltransferases in cell lysates and subcellular fractions.
Structurally, N-alpha-acetyltransferase 60 contains a conserved acetyl-CoA-binding motif and catalytic residues typical of GNAT enzymes. This antibody enables investigation of NAA60's functional role in post-translational regulation and Golgi-associated protein processing.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
