| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NAB2 recombinant protein (Position: D235-Q525) was used as the immunogen for the NAB2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NAB2 Antibody / NGFI-A-binding protein 2 is a anti-NAB2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: NAB2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
NGFI-A-binding protein 2 contains two conserved EGR-binding domains and a repression domain that interacts with the chromatin remodeling machinery. Through these domains, NAB2 forms complexes with EGR transcription factors to fine-tune expression of immediate-early genes involved in cellular differentiation. The NAB2 antibody allows visualization of these interactions in neural and non-neural tissues, facilitating studies on transcriptional control mechanisms that balance growth and differentiation. In the nervous system, NAB2 is essential for Schwann cell maturation and myelin formation, acting downstream of EGR2 signaling.
Mutations or dysregulation of NGFI-A-binding protein 2 have been linked to neuropathies and developmental disorders. NAB2 deficiency in animal models disrupts myelination and peripheral nerve function, underscoring its importance in glial differentiation. The NAB2 antibody supports investigations into these phenotypes by enabling detection of expression patterns during neural development and regeneration. It also serves as a valuable tool for examining NAB2's role in non-neuronal contexts, including cardiac hypertrophy, vascular remodeling, and hormonal signaling.
NAB2 has gained attention in oncology due to the formation of NAB2-STAT6 gene fusions, which drive solitary fibrous tumors. The fusion protein combines the NAB2 repression domain with the STAT6 activation domain, leading to constitutive transcriptional activation. The NAB2 antibody aids in identifying expression changes associated with these fusion events, supporting tumor classification and mechanistic studies. NAB2's broader roles in transcriptional regulation and growth control make it a versatile subject for molecular and pathological research.
The NAB2 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, yielding strong nuclear staining consistent with its transcriptional function.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
