| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NANS recombinant protein (Position: M1-E341) was used as the immunogen for the NANS antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NANS Antibody / Sialic acid synthase is a anti-NANS Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: NANS
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, ELISA
Biological background
Sialic acid synthase is widely expressed in tissues that actively produce glycoproteins, including liver, brain, and immune cells. The enzyme's activity is essential for the biosynthesis of sialylated glycoconjugates, which are critical for neuronal development and immune recognition. The NANS antibody allows researchers to monitor NANS expression levels and subcellular localization, facilitating studies that explore how sialic acid metabolism supports neural growth and synaptic function. In the developing brain, altered sialylation affects axonal targeting and synaptic plasticity, making this enzyme an important target in neurobiology.
Mutations in NANS cause sialic acid synthase deficiency, a rare autosomal recessive disorder characterized by skeletal dysplasia, developmental delay, and metabolic abnormalities. These mutations impair enzyme activity, leading to reduced sialic acid production and accumulation of precursors such as N-acetylmannosamine. The NANS antibody is an essential reagent for diagnosing and studying this deficiency, allowing direct measurement of protein levels and identification of loss-of-function variants. By detecting Sialic acid synthase in cellular and tissue models, researchers can examine how disrupted glycan biosynthesis contributes to disease phenotypes.
Beyond human pathology, Sialic acid synthase plays roles in host-pathogen interactions. Many viruses and bacteria exploit sialic acid residues for attachment and immune evasion. The NANS antibody helps elucidate how modulation of sialic acid synthesis influences infection susceptibility and immune responses. Additionally, this enzyme's regulation under hypoxia and stress conditions highlights its connection to energy metabolism and cellular adaptation.
The NANS antibody is suitable for western blotting, immunofluorescence, and immunohistochemistry. It yields strong cytoplasmic staining consistent with Sialic acid synthase localization.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
