| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NAXE recombinant protein (Position: R46-Q288) was used as the immunogen for the NAXE antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NAXE Antibody / NAD(P)HX epimerase is a anti-NAXE Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Mitochondria), Secreted.
Key elements and design rationale
- Target: NAXE
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
NAD(P)HX epimerase localizes to both the cytosol and mitochondria, reflecting its role in multiple redox compartments. It is essential for the NAD(P)HX repair system, which safeguards the integrity of cellular redox reactions by regenerating functional NADH and NADPH. The NAXE antibody allows researchers to analyze this protective mechanism, revealing how cells maintain coenzyme pools under conditions of heat, oxidative, or metabolic stress. Loss of NAXE function leads to accumulation of toxic NADHX derivatives, disrupting energy metabolism and enzymatic activity.
Mutations in the NAXE gene cause a rare neurometabolic disorder characterized by recurrent encephalopathy and neurodegeneration, particularly following febrile illness. The NAXE antibody supports mechanistic studies into this disease, helping identify tissue-specific expression and enzyme deficiency in patient-derived samples. Defective NAD(P)HX epimerase activity compromises mitochondrial respiration and redox balance, underscoring its essential role in cellular survival.
Beyond its role in repair metabolism, NAD(P)HX epimerase contributes to stress adaptation and antioxidant defense. It ensures a continuous supply of NAD(P)H required for reductive biosynthesis and detoxification pathways. The NAXE antibody supports quantitative analyses of enzyme regulation in models of oxidative stress, aging, and metabolic disorders. Dysregulation of NAD(P)HX repair enzymes has also been linked to impaired neuronal function and energy deficiency syndromes.
The NAXE antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, providing characteristic mitochondrial and cytosolic staining.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
