| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NCDN recombinant protein (Position: F114-K426) was used as the immunogen for the NCDN antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NCDN Antibody / Neurochondrin is a anti-NCDN Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: NCDN
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, FACS, ELISA
Biological background
Neurochondrin, encoded by the NCDN gene on chromosome 12q13.3, is approximately 79 kilodaltons in size and highly conserved among vertebrates. It is enriched in postmitotic neurons and functions as a scaffold linking cell surface receptors with intracellular signaling cascades. The protein associates with calmodulin and modulates calcium-dependent processes critical for neuronal excitability. In animal models, loss of NCDN expression disrupts spatial learning and synaptic signaling, underscoring its role in cognitive function. Furthermore, mutations or altered expression of NCDN have been linked to neurodevelopmental disorders and schizophrenia, highlighting its importance in brain health and disease.
The NCDN antibody is used to study neuronal signaling and synaptic regulation in both developmental and disease contexts. In research applications, it detects Neurochondrin by western blot, immunocytochemistry, and immunohistochemistry. The antibody identifies distinct cytoplasmic localization in neurons, particularly within dendritic arbors, consistent with its role in synaptic modulation. Beyond the brain, Neurochondrin expression has been detected in cardiac and endocrine tissues, suggesting additional regulatory functions outside the nervous system. Proteomic studies have also linked NCDN to cytoskeletal organization and intracellular trafficking networks.
Through its interactions with signaling proteins such as CaMKII and GRM5, Neurochondrin integrates receptor activation with downstream pathways influencing synaptic plasticity and learning. Its modulation of calcium signaling provides a mechanistic link between neurotransmission and neuronal adaptation. Because of these diverse regulatory roles, the NCDN antibody is a valuable reagent for exploring molecular mechanisms underlying cognition, synaptic remodeling, and psychiatric disorders.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
