| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human p47 phox/NCF1 recombinant protein (Position: Q22-R384) was used as the immunogen for the NCF1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NCF1 Antibody / Neutrophil cytosolic factor 1 / p47phox is a anti-NCF1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic.
Key elements and design rationale
- Target: NCF1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, FACS, IF, IHC, WB
Biological background
Neutrophil cytosolic factor 1 becomes phosphorylated upon cellular activation, prompting its translocation to the plasma membrane where it associates with p22phox, gp91phox (CYBB), and other subunits to assemble the active NADPH oxidase complex. This enzymatic system produces superoxide radicals essential for microbial killing. The NCF1 antibody enables detection of this phosphorylation-dependent activation and helps identify conditions that modulate oxidase assembly.
Genetic defects in NCF1 lead to chronic granulomatous disease (CGD), a primary immunodeficiency characterized by recurrent infections due to defective superoxide generation. In such patients, mutations often cause reduced or absent p47phox expression. Using the NCF1 antibody, researchers can distinguish functional versus nonfunctional protein forms, aiding in diagnostic and mechanistic studies of CGD. Beyond innate immunity, Neutrophil cytosolic factor 1 also contributes to redox-dependent signaling in endothelial and neuronal cells, highlighting its importance beyond phagocytes.
The NCF1 antibody is suitable for western blotting, immunoprecipitation, and flow cytometry, where it detects both total and phosphorylated protein forms. Its applications extend to studies examining oxidative stress, cell signaling cascades, and inflammatory pathway regulation. Because NADPH oxidase activity affects vascular tone, apoptosis, and cytokine production, NCF1 has broad physiological relevance. The antibody provides a reliable means to quantify expression changes under oxidative or inflammatory stress.
In oncology and cardiovascular research, aberrant activation of NCF1-containing complexes has been associated with chronic inflammation and tissue damage. Elevated expression may amplify ROS production, promoting DNA damage and tumorigenesis. Conversely, impaired function can weaken immune defense and tissue repair. The NCF1 antibody offered by
By providing consistent detection across multiple platforms, the NCF1 antibody remains an essential reagent for studying oxidative metabolism and immune cell function. Its use continues to expand from immunology into neurobiology and cancer biology, where understanding reactive oxygen mechanisms is increasingly central to therapeutic innovation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
