| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NCLN recombinant protein (Position: Q59-Q563) was used as the immunogen for the NCLN antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NCLN Antibody / Nicalin is a anti-NCLN Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (ER).
Key elements and design rationale
- Target: NCLN
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS, ELISA
Biological background
Nicalin is an integral membrane glycoprotein with several transmembrane domains that anchor it to the ER. It interacts with NOMO (Nodal modulator) and TMEM147 to form a multiprotein assembly that negatively regulates Nodal signaling, a pathway critical for embryonic patterning and left-right asymmetry. The NCLN antibody allows visualization of this protein complex and quantification of Nicalin expression in cultured cells and tissue samples, enabling insights into how ER-based regulatory systems influence developmental signaling.
Beyond embryogenesis, Nicalin maintains normal ER function in adult cells by modulating protein assembly and trafficking. The NCLN antibody is particularly valuable for identifying changes in Nicalin expression during ER stress or unfolded protein response activation. These studies contribute to understanding how misfolded proteins accumulate in disease states such as neurodegeneration, metabolic disorders, and cancer. The localization of Nicalin at the ER membrane and its role in controlling protein export highlight its importance in maintaining proteostasis and cellular viability.
Recent studies suggest that Nicalin may also regulate the biosynthesis of specific receptor and transporter proteins, linking it to neurotransmission and metabolic control. The NCLN antibody supports such investigations by enabling detection of Nicalin across various cell types, including neurons, epithelial cells, and hepatocytes. Its use in immunofluorescence and immunohistochemistry provides high-resolution imaging of ER organization and allows researchers to study how alterations in ER machinery affect overall cell physiology.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
