| Field | Specification |
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| Host | |
| Immunogen | E.coli-derived human NCOA6 recombinant protein (Position: K64-E1475) was used as the immunogen for the NCOA6 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
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| Target | |
| UniProt # |
Overview
NCOA6 Antibody / Nuclear receptor coactivator 6 is a anti-NCOA6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: NCOA6
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
NCOA6 acts as a transcriptional bridge connecting ligand-activated nuclear receptors-including estrogen receptor, thyroid hormone receptor, glucocorticoid receptor, and peroxisome proliferator-activated receptor gamma (PPARG)-to the general transcriptional machinery. Through these interactions, NCOA6 antibody detects a coactivator that is critical for endocrine signaling and metabolism regulation. NCOA6 also cooperates with non-receptor transcription factors such as E2F1, p53, and CREB-binding protein, extending its role beyond hormonal pathways. In metabolic tissues, it contributes to lipid metabolism, gluconeogenesis, and energy homeostasis through regulation of PGC-1alpha and FOXO1 signaling networks.
Structurally, NCOA6 contains multiple protein-binding domains that facilitate interaction with chromatin-modifying enzymes and transcriptional complexes. It functions as a scaffold for the ASCOM complex (ASC-2 complex) that includes MLL family methyltransferases responsible for histone H3 lysine 4 (H3K4) methylation-a mark of active transcription. These modifications alter chromatin accessibility and promote RNA polymerase II recruitment. Alternative splicing of NCOA6 produces several isoforms with tissue-specific regulatory effects.
NCOA6 is expressed ubiquitously but is enriched in liver, reproductive tissues, and endocrine organs. Dysregulation of NCOA6 has been linked to developmental abnormalities, reproductive disorders, and cancers including breast, prostate, and liver carcinoma. Overexpression promotes estrogen-dependent gene transcription and can contribute to hormone-sensitive tumor progression. Genetic studies have also implicated NCOA6 variants in metabolic syndrome, insulin resistance, and congenital heart defects, reflecting its broad physiological significance.
At the chromosomal level, 20q12 amplification encompassing NCOA6 has been identified in several tumor types. Functionally, NCOA6 acts as a coactivator of MYC and E2F transcription factors, enhancing cell proliferation and oncogenic transformation. Conversely, NCOA6 depletion impairs cell-cycle progression and induces apoptosis, indicating its importance for tumor cell survival. Disease-relevant pathways regulated by NCOA6 include oxidative stress response, estrogen signaling, and retinoic acid receptor pathways.
Immunohistochemical staining using NCOA6 antibody shows nuclear localization in hepatocytes, endocrine gland cells, and epithelial tissues. The antibody is a valuable tool for studies of transcriptional regulation, hormone receptor signaling, and chromatin remodeling. NCOA6 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
