| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NCOR1 recombinant protein (Position: H23-Q2075) was used as the immunogen for the NCOR1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NCOR1 Antibody / Nuclear receptor corepressor 1 is a anti-NCOR1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Nuclear.
Key elements and design rationale
- Target: NCOR1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
Functionally, NCOR1 antibody recognizes a large nuclear protein (~270 kDa) that forms complexes with histone deacetylase 3 (HDAC3) and transducin beta-like proteins (TBL1X, TBL1XR1), serving as the catalytic core of the NCOR/SMRT repression complex. NCOR1 regulates gene networks responsive to thyroid hormone, retinoic acid, glucocorticoids, and other ligands by recruiting HDAC3 to promoter regions. Through its receptor-interacting domains, NCOR1 binds unliganded nuclear receptors such as TR, RAR, and RXR to maintain basal transcriptional repression. Upon ligand binding, corepressors are released, and coactivators are recruited to enable gene activation.
In developmental and metabolic regulation, NCOR1 influences adipogenesis, gluconeogenesis, circadian rhythm, and immune cell differentiation. Deletion of NCOR1 in adipose tissue leads to increased mitochondrial activity and energy expenditure, highlighting its role in metabolic homeostasis. In neurons, NCOR1 modulates synaptic plasticity and behavior by repressing neural gene programs. Overexpression or mutation of NCOR1 can disrupt transcriptional balance and contribute to diseases including cancer, metabolic syndrome, and neurodevelopmental disorders. For example, NCOR1 loss correlates with enhanced oncogenic signaling in breast and prostate cancer models.
Structurally, NCOR1 contains multiple repression domains, nuclear receptor interaction motifs (L/IxxI/VI), and coiled-coil regions that enable interaction with HDAC3 and transcription factors. The NCOR1 antibody is widely used in chromatin immunoprecipitation (ChIP), western blotting, and immunofluorescence assays to examine chromatin binding, nuclear localization, and HDAC recruitment. The NCOR1 gene is located on chromosome 17p12 and encodes a 2453-amino acid protein conserved across mammals. It shares functional similarity with NCOR2 (SMRT), though the two exhibit distinct expression profiles and complex-specific functions.
By targeting a master regulator of transcriptional repression, NCOR1 antibody supports research in gene regulation, epigenetics, and signal-dependent chromatin remodeling.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
