| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NCOR2 recombinant protein (Position: A1162-S1362) was used as the immunogen for the NCOR2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NCOR2 Antibody / Nuclear receptor corepressor 2 is a anti-NCOR2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human. Reported localization: Nuclear.
Key elements and design rationale
- Target: NCOR2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, FACS, IF, IHC, ICC, WB
Biological background
Nuclear receptor corepressor 2 contains multiple repression domains, nuclear receptor�interaction motifs, and regions that bind HDAC3. By forming multiprotein complexes, NCOR2 promotes chromatin condensation and transcriptional silencing through histone deacetylation. The NCOR2 antibody enables visualization of this protein in nuclear extracts and tissue samples, supporting studies that examine gene regulation by thyroid hormone, retinoic acid, glucocorticoid, and estrogen receptors. Through these interactions, NCOR2 modulates key pathways governing metabolism, development, and circadian rhythm.
Mutations or altered expression of NCOR2 disrupt hormonal and metabolic balance, contributing to endocrine and developmental disorders. In addition, aberrant nuclear receptor corepressor 2 function has been linked to oncogenesis, particularly in breast and prostate cancers, where loss of repression leads to hormone-independent growth. The NCOR2 antibody is used to assess expression levels and nuclear localization patterns, helping identify correlations between corepressor dysfunction and tumor progression. By supporting detection of NCOR2 in cancer tissues, this antibody aids in understanding transcriptional misregulation associated with tumorigenesis.
Beyond hormone receptor signaling, NCOR2 coordinates repression of inflammatory and immune genes by interacting with transcription factors such as NF-?B and STATs. It participates in maintaining immune tolerance and suppressing chronic inflammation. The NCOR2 antibody enables functional studies into these processes, revealing how corepressor complexes shape immune gene expression. In the central nervous system, NCOR2 also influences neuronal differentiation and synaptic plasticity, underscoring its role as a global regulator of chromatin dynamics.
The NCOR2 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry, exhibiting strong nuclear staining consistent with its role as a transcriptional corepressor.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
