| Field | Specification |
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| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human NCR3 was used as the immunogen for the NCR3 antibody. |
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Overview
NCR3 Antibody / Natural cytotoxicity receptor 3 is a anti-NCR3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: NCR3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC
Biological background
Functionally, NCR3 antibody identifies a 190-amino-acid type I transmembrane protein containing a single extracellular immunoglobulin-like domain responsible for ligand recognition. NCR3 binds to several cellular and pathogen-derived ligands, including B7-H6, BAG6, and viral hemagglutinin, triggering NK cell activation and cytotoxic granule release. Engagement of NCR3 leads to downstream signaling through the ITAM-containing adaptor protein CD3 zeta, resulting in cytokine production (IFN-gamma, TNF-alpha) and target cell killing. Beyond its role in NK cells, NCR3 expression has also been detected in subsets of T cells and innate lymphoid cells, where it contributes to immune modulation and inflammation.
The NCR3 gene is located on chromosome 6p21.3 within the major histocompatibility complex (MHC) region. Expression is tightly regulated by cytokines such as IL-2 and IL-15, which promote NK cell activation and expansion. NCR3 activity is balanced by interactions with both activating and inhibitory receptors to maintain immune homeostasis.
Pathologically, dysregulated NCR3 signaling influences susceptibility to infections, tumor immune evasion, and autoimmune conditions. Reduced receptor expression or function can impair NK cell cytotoxicity, while overactivation contributes to tissue inflammation and autoimmunity. Elevated NCR3 activity has been reported in various cancers and viral infections, highlighting its importance as a biomarker for immune activation and immunotherapy potential. Research using NCR3 antibody supports studies in NK cell biology, tumor immunology, and antiviral defense.
NCR3 antibody is validated for use in relevant research applications to study receptor-mediated cytotoxicity and immune signaling.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
