| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human B7H6/NCR3LG1 recombinant protein (Position: H85-D386) was used as the immunogen for the NCR3LG1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NCR3LG1 Antibody / B7-H6 / Natural cytotoxicity triggering receptor 3 ligand 1 is a anti-NCR3LG1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cell membrane.
Key elements and design rationale
- Target: NCR3LG1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
Natural cytotoxicity triggering receptor 3 ligand 1 is a type I transmembrane protein belonging to the B7 family of immune regulatory molecules. It is normally absent from healthy tissues but is induced by cellular stress, transformation, or infection. The NCR3LG1 antibody enables researchers to detect B7H6 expression on tumor cells, identifying targets for immune recognition and clearance. Because it acts as a danger signal for NK cell activation, B7H6 is a focus of research in cancer immunotherapy and immune checkpoint modulation.
Expression of B7H6 has been reported in various tumors, including leukemia, lymphoma, melanoma, and carcinomas of the lung and ovary. The NCR3LG1 antibody supports studies that examine the relationship between B7H6 expression and tumor immune evasion. Tumor cells may shed soluble B7H6 into the extracellular environment, impairing NK cell function. Detection using the NCR3LG1 antibody allows quantification of both membrane-bound and soluble forms, offering insights into immune escape mechanisms and therapeutic targeting opportunities.
In the context of infection, Natural cytotoxicity triggering receptor 3 ligand 1 acts as a stress-induced molecule upregulated by viral or bacterial pathogens, promoting immune activation. The NCR3LG1 antibody supports investigation of these innate immune responses, clarifying how NKp30�B7H6 interactions contribute to early pathogen clearance. Additionally, the ligand's selective expression profile makes it an attractive target for engineered NK cell or bispecific antibody therapies designed to enhance antitumor immunity.
The NCR3LG1 antibody is validated for flow cytometry, immunofluorescence, and western blotting, producing distinct membrane staining in positive cell lines.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
