| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NDST1 recombinant protein (Position: 40-87) was used as the immunogen for the NDST1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NDST1 Antibody / N-deacetylase/N-sulfotransferase 1 is a anti-NDST1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Western blot (WB) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: NDST1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, WB
Biological background
NDST1 is encoded by the NDST1 gene located on human chromosome 5q33.1. The protein is approximately 882 amino acids long and resides in the lumen of the Golgi apparatus, where it acts early in heparan sulfate chain modification. NDST1 belongs to the heparan sulfate modification enzyme family, working in concert with other sulfotransferases and epimerases to establish specific sulfation patterns that control the binding affinity of heparan sulfate to growth factors, morphogens, and cytokines.
The NDST1 antibody detects a 100 kilodalton band by western blot and demonstrates perinuclear Golgi localization under immunofluorescence microscopy. NDST1 activity is essential for embryonic development, vascular formation, and neural patterning. Through its modification of heparan sulfate, NDST1 regulates key signaling pathways including FGF, Wnt, Hedgehog, and BMP. Loss or mutation of NDST1 disrupts heparan sulfate composition, leading to developmental abnormalities and impaired growth factor signaling.
In cancer, NDST1 expression is often dysregulated, resulting in altered extracellular matrix structure and aberrant cell signaling. Overexpression enhances tumor invasiveness by modifying cell-surface heparan sulfate, while reduced NDST1 activity interferes with angiogenesis. It also contributes to inflammation by modulating chemokine gradients and leukocyte migration.
Because NDST1 determines the sulfation code of heparan sulfate, it serves as a critical regulator of cell communication and extracellular architecture.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
