| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human NDUFB11 was used as the immunogen for the NDUFB11 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NDUFB11 Antibody / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11 is a anti-NDUFB11 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Mitochondria).
Key elements and design rationale
- Target: NDUFB11
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF
Biological background
Structurally, NDUFB11 is a highly conserved, hydrophobic protein of approximately 17 kilodaltons, localized to the mitochondrial inner membrane with one transmembrane helix. It anchors and stabilizes neighboring subunits within the peripheral arm of Complex I, enabling efficient electron flow and coupling of redox reactions to proton translocation. Loss or mutation of NDUFB11 disrupts complex assembly, leading to impaired respiratory chain activity and reduced ATP generation.
The NDUFB11 antibody is widely used in mitochondrial biology, metabolic disease, and bioenergetics research to study oxidative phosphorylation and Complex I organization. Western blot analysis detects a 17 kilodalton band corresponding to NDUFB11, while immunofluorescence reveals characteristic mitochondrial localization that colocalizes with markers such as COX IV. This antibody provides a reliable means of evaluating mitochondrial function, biogenesis, and assembly defects in various models.
Pathogenic variants in NDUFB11 cause X-linked mitochondrial Complex I deficiency, often presenting as hypertrophic cardiomyopathy, encephalopathy, or lactic acidosis. Altered NDUFB11 expression has also been observed in cancers and neurodegenerative disorders, where mitochondrial dysfunction contributes to disease progression. The NDUFB11 antibody enables detection of expression changes, assessment of mitochondrial health, and verification of respiratory chain assembly status.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
