| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NF-M/NEFM recombinant protein (Position: Q125-D916) was used as the immunogen for the NEFM antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NEFM Antibody / NF-M / Neurofilament medium is a anti-NEFM Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, cytoskeleton.
Key elements and design rationale
- Target: NEFM
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, IP, FACS, ELISA
Biological background
Functionally, NEFM antibody identifies a 916-amino-acid cytoskeletal protein containing an alpha-helical coiled-coil rod domain and an extensive C-terminal tail rich in phosphorylation sites. NEFM contributes to the structural scaffolding of axons, determining their caliber and conduction velocity. Its phosphorylation regulates filament spacing and interaction with other cytoskeletal components, thereby influencing axonal transport and mechanical stability.
The NEFM gene is located on chromosome 8p21.2 and is highly expressed in neurons of the central and peripheral nervous systems. NEFM is particularly abundant in large myelinated axons, where it associates with microtubules and neurofilament light and heavy chains to form cross-linked filamentous arrays. During development, NEFM expression increases as neurons mature, supporting long-range axonal projection and signal transmission.
In pathology, NEFM serves as a biomarker of axonal injury and neurodegeneration. Elevated NEFM levels are detected in cerebrospinal fluid and plasma of patients with amyotrophic lateral sclerosis (ALS), Alzheimer's disease, and traumatic brain injury, reflecting axonal breakdown. In experimental models, altered NEFM phosphorylation contributes to impaired axonal transport and neurofilament aggregation, features commonly seen in neurodegenerative diseases.
NEFM antibody is widely used in neuroscience, neuropathology, and cytoskeletal biology research. It is suitable for immunohistochemistry, immunofluorescence, and western blotting to detect NF-M localization and integrity in neuronal tissues. This antibody supports studies of axonal structure, neurofilament organization, and neural injury responses. In translational studies, NEFM detection provides a molecular readout of neuroaxonal damage and regeneration.
Structurally, NEFM assembles into 10-nm filaments with a coiled-coil backbone and phosphorylated C-terminal sidearms that control filament spacing and cytoskeletal elasticity.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
