| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NEK11 recombinant protein (Position: E72-Q630) was used as the immunogen for the NEK11 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NEK11 Antibody / Serine/threonine-protein kinase Nek11 is a anti-NEK11 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: NEK11
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Functionally, NEK11 antibody identifies a 645-amino-acid nuclear and cytoplasmic protein that becomes activated through phosphorylation in response to DNA damage. NEK11 phosphorylates and promotes the degradation of CDC25A phosphatase, inhibiting activation of CDK1-cyclin B and preventing premature entry into mitosis. By coupling checkpoint signaling to cell cycle control, NEK11 preserves genomic stability during replication stress and DNA repair. It also interacts with other checkpoint kinases such as CHK1 and PLK1, forming a regulatory network that ensures proper mitotic timing.
The NEK11 gene is located on chromosome 3q22.1 and is expressed in proliferative tissues including testis, thymus, and intestinal epithelium. Expression levels are highest in S and G2 phases, consistent with its role in cell cycle regulation. NEK11 activity integrates stress signals with checkpoint arrest and recovery mechanisms.
Pathologically, loss or mutation of NEK11 leads to genomic instability, defective DNA repair, and tumorigenesis. Downregulation is observed in colorectal, liver, and lung cancers, where impaired checkpoint function contributes to uncontrolled proliferation. Conversely, elevated NEK11 expression may sensitize tumor cells to genotoxic therapy by prolonging checkpoint arrest. Research using NEK11 antibody supports studies in DNA damage response, checkpoint regulation, and cancer biology.
NEK11 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect checkpoint kinases.
Structurally, Serine/threonine-protein kinase Nek11 contains an N-terminal catalytic kinase domain with a conserved lysine essential for ATP binding and a C-terminal regulatory region mediating protein-protein interactions. Its activation loop undergoes autophosphorylation to enhance catalytic activity following DNA damage. This antibody enables analysis of NEK11's role in coordinating checkpoint signaling and maintaining genomic stability.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
