| Field | Specification |
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| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human Nesprin3 was used as the immunogen for the Nesprin 3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Nesprin 3 Antibody / SYNE3 is a anti-SYNE3 Rabbit antibody Recombinant Rabbit Monoclonal clone 30S39 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: SYNE3
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30S39, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
The LINC complex plays a central role in transmitting mechanical forces between the cytoskeleton and the nucleus. By tethering the nuclear envelope to intermediate filament networks, Nesprin 3 contributes to nuclear anchorage during migration, differentiation, and mitosis. Research using Nesprin 3 antibody has shown that loss of this protein disrupts nuclear shape and mechanosensing, leading to altered transcriptional responses. These findings emphasize the importance of Nesprin 3 in structural integrity and gene regulation.
Dysfunction of Nesprin proteins, including Nesprin 3, has been linked to muscular dystrophies, cardiomyopathies, and neurological disorders. While most studies have focused on Nesprin 1 and Nesprin 2, evidence suggests that Nesprin 3 contributes to nuclear envelope stability and tissue homeostasis. Studies using Nesprin 3 antibody have associated its altered expression with cancer progression, where changes in nuclear deformability affect invasion and metastasis. These roles highlight the significance of SYNE3 in both health and disease.
Nesprin 3 antibody is widely applied in western blotting, immunofluorescence, and immunohistochemistry. Western blotting demonstrates isoform expression, immunofluorescence shows its localization at the nuclear envelope, and immunohistochemistry reveals its distribution in tissues such as muscle, epithelium, and nervous system. Functional studies have employed Nesprin 3 antibody in knockdown and rescue experiments, linking its absence to nuclear detachment and impaired motility.
By providing validated Nesprin 3 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
