| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NETO1 recombinant protein (Position: R5-R514) was used as the immunogen for the NETO1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NETO1 Antibody / Neuropilin and tolloid-like protein 1 is a anti-NETO1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: NETO1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
NETO1 is encoded by the NETO1 gene located on human chromosome 18q22.1. The protein is approximately 533 amino acids long and contains two extracellular CUB domains, a low-density lipoprotein receptor-like domain, a single transmembrane helix, and a short cytoplasmic tail. NETO1 is predominantly expressed in the hippocampus, cortex, and cerebellum, where it localizes to postsynaptic membranes and interacts with KAR subunits such as GluK2 and GluK5.
The NETO1 antibody detects a 63 kilodalton protein by western blot and shows punctate synaptic staining in neurons. NETO1 enhances the stability and functional diversity of KARs by modulating desensitization kinetics and surface expression. It contributes to long-term potentiation and synaptic scaling, processes underlying memory and learning. Mice lacking NETO1 display deficits in synaptic plasticity and spatial learning, confirming its critical role in excitatory neurotransmission.
Beyond the brain, NETO1 may participate in developmental signaling and axonal growth. Dysregulation or mutation of NETO1 has been associated with autism spectrum disorder, schizophrenia, and cognitive impairment. By shaping receptor signaling and synaptic strength, NETO1 maintains proper excitatory balance within neural circuits.
As a neuron-specific regulatory subunit of kainate receptors, NETO1 is a key molecule for studying excitatory synaptic physiology and plasticity.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
