| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NETO2 recombinant protein (Position: I23-F525) was used as the immunogen for the NETO2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NETO2 Antibody / Neuropilin and tolloid-like protein 2 is a anti-NETO2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: NETO2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
NETO2 is encoded by the NETO2 gene located on human chromosome 16q12.1. The protein is approximately 525 amino acids in length and contains two extracellular CUB domains, a low-density lipoprotein receptor-like domain, a transmembrane segment, and a short cytoplasmic tail. It localizes to the postsynaptic membrane in neurons, where it interacts with KAR subunits such as GluK1 and GluK2 to regulate receptor assembly and surface expression.
The NETO2 antibody detects a 62 kilodalton protein by western blot and reveals punctate synaptic staining under immunofluorescence microscopy. NETO2 enhances the functional diversity of KARs by altering desensitization rates and gating properties, shaping excitatory signaling in neural circuits. It is required for normal synaptic strength and contributes to processes like long-term potentiation and synaptic scaling.
Loss of NETO2 leads to impaired excitatory transmission, altered neuronal connectivity, and behavioral abnormalities, including deficits in learning and memory. NETO2 is expressed in multiple brain regions, including the hippocampus and cortex, and its dysregulation has been implicated in autism spectrum disorders and schizophrenia. Outside the brain, NETO2 may participate in neuronal migration and development of sensory pathways.
Because NETO2 serves as a critical regulator of excitatory synapse function, it provides valuable insight into glutamate receptor modulation and synaptic homeostasis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
