| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NEU3 recombinant protein (Position: H36-D414) was used as the immunogen for the NEU3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NEU3 Antibody / Sialidase 3 is a anti-NEU3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: NEU3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, NEU3 antibody identifies a 428-amino-acid enzyme that preferentially acts on gangliosides such as GM3, GD1a, and GT1b, catalyzing their desialylation. NEU3 activity alters the composition of lipid rafts, influencing membrane receptor function and downstream signaling. It modulates the activity of growth factor receptors, including EGFR and integrins, by affecting their sialic acid content and clustering at the plasma membrane. Through these mechanisms, NEU3 participates in the regulation of cell proliferation, adhesion, and migration.
The NEU3 gene is located on chromosome 11q13.5 and encodes a glycosidase that is anchored to the plasma membrane via hydrophobic domains. NEU3's enzymatic activity contributes to ganglioside catabolism and recycling, which are critical for maintaining lipid membrane homeostasis. Dysregulation of NEU3 expression has been linked to oncogenesis, as elevated levels promote tumor cell survival, motility, and resistance to apoptosis. Conversely, NEU3 deficiency impairs receptor-mediated signaling and disrupts ganglioside balance in neuronal and epithelial cells.
In cancer biology, NEU3 overexpression enhances epidermal growth factor receptor (EGFR) signaling by desialylating GM3, a natural EGFR inhibitor. This results in sustained receptor activation and downstream MAPK and PI3K/AKT pathway stimulation. NEU3 also influences integrin-mediated adhesion and migration, contributing to metastasis. Beyond oncology, NEU3 is involved in immune modulation, neuronal signaling, and muscle differentiation. It also regulates autophagy by modulating membrane glycosphingolipid composition.
NEU3 antibody is widely used in glycobiology, cancer research, and neurobiology to analyze sialidase function and membrane signaling. It supports applications including immunoblotting, immunocytochemistry, and enzyme localization studies. NEU3 expression profiling provides insight into tumor progression, inflammatory signaling, and metabolic disorders. The enzyme's surface localization makes it a potential therapeutic target for modulating receptor activity and glycosphingolipid metabolism.
Structurally, NEU3 contains conserved catalytic motifs typical of sialidases and a hydrophobic transmembrane anchor that facilitates membrane association. It functions as a monomer and is regulated by pH and substrate availability.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
