| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Neuronatin/NNAT was used as the immunogen for the Neuronatin antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Neuronatin Antibody / NNAT is a anti-NNAT Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: NNAT
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): IHC, IF, FACS
Biological background
Neuronatin is encoded by the NNAT gene on human chromosome 20q11.23. Two isoforms, NNAT-alpha and NNAT-beta, arise from alternative splicing and encode proteins of approximately 81 and 70 amino acids, respectively. Both isoforms are hydrophobic, integral membrane proteins localized to the endoplasmic reticulum. Structurally, Neuronatin shares sequence similarity with phospholamban and sarcolipin, suggesting a conserved role in modulating the sarco/endoplasmic reticulum calcium ATPase (SERCA) pump. Through this interaction, Neuronatin influences calcium flux and storage, which are critical for neuronal excitability and hormone secretion.
The Neuronatin antibody reveals protein expression during embryonic and postnatal development in the brain, spinal cord, pituitary gland, and pancreas. Immunostaining shows localization within neuronal soma and processes, as well as in pancreatic beta cells, where Neuronatin contributes to insulin secretion. Western blot analysis typically detects a low molecular weight band corresponding to one or both isoforms. Functional studies demonstrate that Neuronatin enhances intracellular calcium release and supports the differentiation of neural progenitor cells by activating calcium-dependent transcriptional programs.
Beyond its developmental functions, Neuronatin has been implicated in metabolic regulation and energy homeostasis. It modulates endoplasmic reticulum stress responses and may act as a mediator between nutrient sensing and neuronal metabolism. Dysregulation of NNAT expression has been associated with obesity, type 2 diabetes, and neurological disorders such as epilepsy and schizophrenia. Aberrant imprinting or epigenetic silencing of the paternal NNAT allele has been reported in human cancers, indicating a potential role in tumor suppression and differentiation control.
Neuronatin expression persists in adult tissues with high metabolic demand, including hypothalamic nuclei, skeletal muscle, and endocrine pancreas. It functions as a calcium-dependent signal amplifier that adjusts energy utilization and cellular excitability. In neurons, NNAT influences synaptic development and may interact with signaling proteins that control axonal growth.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
