| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, GFP (constitutively expressed), GFP-P2A-Puro (constitutively expressed), Puromycin, RFP (constitutively expressed), RFP-P2A-BSD (constitutively expressed) |
| Shipping | |
| Species |
Background
NF-κB is a family of inducible transcription factors, including the subunits encoded by NFKB1 (p50) and RELA (p65), that control inflammation, innate and adaptive immunity, cell survival, and stress responses. In resting cells, NF-κB dimers are held inactive in the cytoplasm by IκB inhibitor proteins. Stimulation by cytokines such as TNF and IL-1, pathogen-associated molecules, or other stressors activates the IKK complex, which phosphorylates IκB and triggers its degradation. The freed NF-κB dimers translocate to the nucleus and bind κB DNA elements to drive transcription of inflammatory and pro-survival genes. Dysregulated NF-κB signaling underlies chronic inflammation, autoimmune disease, and cancer.
Product Description & Applications
The NF-κB Reporter Lentivirus is a lentiviral reporter system that provides a sensitive fluorescent (GFP/RFP) or luminescent readout of NF-κB pathway activity in human and mouse cells. The construct contains a minimal TATA promoter, tandem κB DNA-binding elements, and an optimized mini-enhancer that amplifies NF-κB-dependent reporter expression for strong signal-to-noise. An EF1a-driven selection marker (puromycin or blasticidin) enables easy generation of stable reporter cell lines. Supplied as high-titer, VSV-G-pseudotyped, serum-free particles purified by PEG precipitation and sucrose gradient centrifugation, the system transduces a broad range of cell types, including difficult-to-transfect primary and thawed cells, and supports readout by fluorescence microscopy, flow cytometry, or luminometry for inflammation, immune signaling, and pathway-modulator studies.
About This Product
This reporter lentivirus places a BFP, Firefly Luc, GFP, GFP-P2A-FLuc, Luc, Renilla Luc, RFP, RFP-P2A-FLuc, SEAP, ZsGreen reporter gene under the control of tandem consensus response elements specific for the NFκB signaling pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, GFP (constitutively expressed), GFP-P2A-Puro (constitutively expressed), Puromycin, RFP (constitutively expressed), RFP-P2A-BSD (constitutively expressed)) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
DNA methyltransferase 1 regulates epithelial cell functions in corneal and eyelid development.
Christianto A, Mongan M, Xiao B, Wang Q, et al.
Molecular Vision, 2025.
Product(s) used: LTV-0002, LTV-0011
Usage: Multiple pathway luciferase reporter lentiviruses (LipExoGen) including NF-κB and TCF/LEF reporters used to establish stable Dnmt1 F/F corneal epithelial reporter cells; Cre-mediated Dnmt1 deletion assessed for downstream pathway effects.
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