| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, GFP (constitutively expressed), Puromycin, RFP (constitutively expressed) |
| Shipping | |
| Species |
Background
NFAT (nuclear factor of activated T cells) transcription factors, including NFATc1 and NFATc2, are key regulators of immune cell activation and other calcium-dependent processes. Engagement of antigen or growth factor receptors raises intracellular calcium, activating the phosphatase calcineurin, which dephosphorylates cytoplasmic NFAT and drives its nuclear translocation. In the nucleus, NFAT frequently cooperates with AP-1 at composite sites, such as the antigen response element of the human IL-2 promoter, to induce cytokine and activation genes. NFAT signaling governs T cell activation, immune responses, and processes including cardiac and skeletal development, making it a central readout in immunology and cell signaling research.
Product Description & Applications
The NFAT Reporter Lentivirus places a reporter gene under the control of tandem NFAT/AP-1 composite sites derived from the human IL-2 promoter, providing a sensitive readout of NFAT activity in mammalian cells. Reporter options include fluorescent (GFP, RFP, BFP), luminescent (firefly and Renilla luciferase), and combined GFP- or RFP-P2A-firefly luciferase formats. An EF1-alpha promoter drives a selectable drug marker (puromycin, blasticidin) or constitutive fluorescent protein for stable cell line generation.
The particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary T cells and cryopreserved cultures. Applications include monitoring T cell activation, calcium/calcineurin signaling, and screening immunomodulatory compounds.
About This Product
This reporter lentivirus places a BFP, Firefly Luc, GFP, GFP-P2A-FLuc, Luc, Renilla Luc, RFP, RFP-P2A-FLuc reporter gene under the control of tandem consensus response elements specific for the Calcium/NFAT pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, GFP (constitutively expressed), Puromycin, RFP (constitutively expressed)) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
