| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human NFE2 was used as the immunogen for the NFE2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NFE2 Antibody / Nuclear factor erythroid 2 is a anti-NFE2 Rabbit antibody Recombinant Rabbit Monoclonal clone 31N33 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP) with listed reactivity in Human.
Key elements and design rationale
- Target: NFE2
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31N33, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF, IP
Biological background
The p45 subunit of NFE2 binds to antioxidant response elements and regulates transcription of genes involved in heme biosynthesis, hemoglobin assembly, and detoxification of reactive oxygen species. In megakaryocytes, NFE2 is required for proplatelet formation and platelet release. Detection of this factor using NFE2 antibody helps identify regulatory pathways that maintain normal blood development and function.
Mutations or dysregulation of NFE2 are implicated in hematological disorders, including myeloproliferative neoplasms. Overexpression of NFE2 has been reported in polycythemia vera and essential thrombocythemia, linking the transcription factor to abnormal erythrocyte and platelet production. Research employing NFE2 antibody has revealed roles in leukemogenesis and abnormal megakaryopoiesis. Because of these connections, NFE2 is studied as a potential therapeutic target in blood cancers.
NFE2 antibody can be applied in western blotting, immunohistochemistry, and electrophoretic mobility shift assays. Western blotting confirms protein expression in erythroid cell lines, while immunohistochemistry highlights tissue-specific nuclear localization. In gene regulation studies, chromatin immunoprecipitation with NFE2 antibody allows mapping of target promoters controlled by this transcription factor.
NFE2 is also relevant in oxidative stress research. Its regulation of antioxidant genes links it to cellular defense mechanisms against free radicals and environmental toxins. Researchers using NFE2 antibody can explore how transcriptional control intersects with oxidative signaling and stress adaptation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
