| Field | Specification |
|---|---|
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, Puromycin |
| Shipping | |
| Species |
Background
NFE2 (nuclear factor, erythroid 2, 45 kDa subunit) is a basic leucine zipper transcription factor essential for hematopoietic differentiation. It forms heterodimers with small MAF proteins, such as MAF-G, that bind NFE2 DNA elements and regulate gene expression. NFE2 is required for normal erythroid maturation and is a critical regulator of megakaryocyte development and platelet production. Loss of NFE2 function disrupts platelet biogenesis, while dysregulated NFE2 activity has been linked to myeloproliferative disorders. Through its control of erythroid and megakaryocytic gene programs, NFE2-dependent transcription is an informative readout in hematopoiesis and blood cell biology research.
Product Description & Applications
The NFE2 Reporter Lentivirus is a transcription-factor reporter system that provides a sensitive fluorescent or luminescent readout of human or mouse NFE2 activity. The construct uses consensus NFE2 DNA-binding elements derived from the PBGD promoter, coupled to a minimal promoter, to drive a reporter gene, while a constitutively expressed selection marker supports stable cell line generation. Reporter options include fluorescent proteins and luciferase, with readout by fluorescence microscopy, flow cytometry, or luminometry.
The system is suited to studying NFE2 activity in erythroid and megakaryocytic differentiation, platelet biology, and related hematologic processes. Particles are purified by PEG precipitation and sucrose gradient centrifugation, enabling effective transduction of difficult-to-transfect primary and thawed cells.
About This Product
This reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the NFE2 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
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