| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NFIA recombinant protein (Position: V172-Q233) was used as the immunogen for the NFIA antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NFIA Antibody / Nuclear factor 1 A-type is a anti-NFIA Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: NFIA
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
NFIA is encoded by the NFIA gene located on human chromosome 1p31.3. The protein contains a conserved N-terminal DNA-binding domain responsible for dimerization and sequence-specific binding, along with a C-terminal transactivation/repression region that interacts with other transcriptional regulators. NFIA forms homo- and heterodimers with other NFI family members (NFIB, NFIC, NFIX), allowing combinatorial control of target genes. Studies in model organisms have shown that loss of NFIA disrupts gliogenesis, leading to abnormal brain structure and impaired glial differentiation.
Expression profiling reveals that NFIA is abundant in glial progenitor cells and astrocytes, while its expression declines as neurons mature. Western blot analysis using NFIA antibody typically identifies a band near 54-60 kDa (unphosphorylated form), corresponding to the full-length transcription factor. Immunohistochemistry reveals strong nuclear localization in developing brain regions, spinal cord, and other tissues undergoing differentiation. NFIA cooperates with SOX9, STAT3, and other transcriptional factors to regulate astrocytic gene programs, such as those encoding GFAP and S100B.
In addition to its role in neurodevelopment, NFIA participates in the regulation of kidney morphogenesis, adipogenesis, and hematopoietic stem cell maintenance. Dysregulation or mutation of NFIA has been linked to developmental delay, macrocephaly, and agenesis of the corpus callosum in human syndromic cases.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
