| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NFS1 recombinant protein (Position: R107-L356) was used as the immunogen for the NFS1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NFS1 Antibody / Cysteine desulfurase is a anti-NFS1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: NFS1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IP, FACS, ELISA
Biological background
Structurally, NFS1 is a pyridoxal phosphate (PLP)-dependent enzyme that functions as a homodimer. It forms a core part of the Fe-S cluster assembly complex with ISCU (scaffold protein), ISD11, ACP (acyl carrier protein), and FXN (Frataxin). NFS1 donates sulfur from cysteine via a persulfide intermediate, which is transferred to ISCU for subsequent cluster assembly. The protein belongs to the cysteine desulfurase family, conserved across prokaryotes and eukaryotes. Co-localization studies show NFS1 residing in the mitochondrial matrix, where it coexists with other Fe-S biosynthesis factors such as ISCU and FXN.
Functionally, NFS1 supports the biogenesis of Fe-S cluster-dependent enzymes including aconitase, succinate dehydrogenase, and DNA polymerase complexes. It plays a key role in oxidative phosphorylation, the tricarboxylic acid (TCA) cycle, and cellular redox homeostasis. In addition to mitochondrial roles, NFS1 also provides sulfur for cytosolic Fe-S protein assembly via the iron-sulfur cluster export machinery. Known interaction partners include ISD11, FXN, and NUBP2, which together ensure the stability and efficiency of sulfur transfer and cluster formation.
Dysregulation or mutation of NFS1 disrupts Fe-S cluster biogenesis and results in severe mitochondrial dysfunction. Deficiency causes combined oxidative phosphorylation deficiency 20 (COXPD20), characterized by lactic acidosis, developmental delay, and myopathy. In cancer, altered NFS1 expression influences metabolic reprogramming and redox balance. Pathway associations include iron-sulfur cluster biosynthesis, oxidative phosphorylation, and mitochondrial sulfur metabolism. Expression of NFS1 is particularly high in metabolically active tissues such as heart, liver, and skeletal muscle.
The NFS1 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
