| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NFU1 recombinant protein (Position: Q42-P254) was used as the immunogen for the NFU1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NFU1 Antibody / HIRA-interacting protein 5 / HIRIP5 is a anti-NFU1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, Mitochondria.
Key elements and design rationale
- Target: NFU1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
Iron-sulfur cluster scaffold protein NFU1 acts downstream of the core Fe-S assembly machinery (ISC system) and transfers preassembled clusters to specific target proteins, including lipoic acid synthetase and succinate dehydrogenase. It contains a conserved C-terminal cysteine-rich domain that binds transient Fe-S clusters before delivery to recipient apoproteins. The NFU1 antibody enables localization and expression analysis, showing strong mitochondrial matrix staining consistent with its chaperone-like function in cluster transfer.
Mutations in the NFU1 gene cause Multiple Mitochondrial Dysfunction Syndrome 1 (MMDS1), a severe autosomal recessive disorder characterized by infantile encephalopathy, lactic acidosis, and respiratory-chain deficiency. The NFU1 antibody supports investigations into the molecular mechanisms of MMDS1 by allowing detection of protein loss or misfolding in patient cells. Loss of functional NFU1 impairs Fe-S enzyme assembly, reducing activity of complexes II and lipoic acid�dependent dehydrogenases, ultimately compromising cellular energy metabolism.
Beyond Fe-S cluster assembly, Iron-sulfur cluster scaffold protein NFU1 contributes to the maintenance of redox balance and the regulation of reactive oxygen species. The NFU1 antibody supports quantitative studies in models of oxidative stress, aging, and mitochondrial dysfunction. Reduced NFU1 expression has been associated with altered mitochondrial respiration and metabolic imbalance in cancer and neurodegeneration.
The NFU1 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, producing strong mitochondrial staining.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
