| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human Entactin/NID1 recombinant protein (Position: R31-H1209) was used as the immunogen for the NID1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NID1 Antibody / Nidogen 1 / Entactin is a anti-NID1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse.
Key elements and design rationale
- Target: NID1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, FACS, ELISA
Biological background
Nidogen 1 contains globular domains separated by flexible segments that adapt to different extracellular partners. Its localization along the basement membrane enables crosstalk between matrix and cells, influencing receptor signaling through integrins and dystroglycan. Depletion in animal models disrupts organogenesis and compromises barrier function in kidney and vasculature, highlighting the protein's structural and signaling roles. Because matrix composition tunes growth factor availability, Nidogen 1 also contributes to gradient formation and ligand presentation within tissue microenvironments.
Researchers use NID1 antibody / Entactin in immunohistochemistry to delineate basement membranes in skin, kidney, lung, and tumor samples, providing clear borders for morphometric analyses and pathology scoring. In immunoblotting, the reagent tracks expression during development or matrix remodeling, while immunofluorescence co-localization with laminin and collagen IV reveals network integrity and continuity. In vitro, combining the antibody with matrix assembly assays clarifies how Nidogen 1 scaffolds influence cell adhesion, migration, and polarity under defined conditions.
The NID1 antibody / Entactin from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
