| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NIP7 recombinant protein (Position: E14-T180) was used as the immunogen for the NIP7 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NIP7 Antibody / Nucleolar pre-rRNA processing protein NIP7 is a anti-NIP7 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse.
Key elements and design rationale
- Target: NIP7
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
NIP7 is encoded by the NIP7 gene located on human chromosome 18q12.1. The protein is approximately 20 kilodaltons in size and localized predominantly in the nucleolus, where it associates with pre-ribosomal particles. Structurally, NIP7 contains an Sm-like fold and RNA-binding motifs enabling it to interact with ribosomal RNA precursors and small nucleolar RNAs. It forms complexes with NOP8 and RRP12, essential for the processing of 20S pre-rRNA and export of pre-40S subunits to the cytoplasm. These interactions make NIP7 a critical factor in maintaining ribosome synthesis efficiency.
The NIP7 antibody is valuable for detecting nucleolar proteins by western blot or immunocytochemistry. Western blot typically shows a distinct band at approximately 25 kilodaltons, while immunofluorescence highlights punctate nucleolar localization that co-stains with fibrillarin or nucleolin. Depletion of NIP7 disrupts 18S rRNA maturation, leading to defective ribosome assembly and impaired cell growth. Beyond ribosome synthesis, NIP7 may participate in broader RNA processing pathways, linking ribosome production to cell cycle progression and metabolic control.
NIP7 is evolutionarily conserved from yeast to humans, emphasizing its fundamental role in RNA biology. Altered expression has been noted in certain cancers and stress conditions that perturb ribosome biogenesis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
