| Field | Specification |
|---|---|
| Expression System | |
| Form | Liquid |
| Product Type | |
| Reporter | |
| Storage | |
| Target |
Scientific Background
Nipah Virus (NiV) is a highly pathogenic paramyxovirus (family Paramyxoviridae, genus Henipavirus) classified as a Biosafety Level 4 (BSL-4) agent and a WHO priority pathogen. NiV causes fatal encephalitis and respiratory disease in humans, with case fatality rates ranging from 40–75%. The virus uses ephrin B2 and B3 as cell entry receptors, mediated by the viral attachment glycoprotein (G) and fusion protein (F). Because work with live NiV requires BSL-4 containment, the development of safe surrogate systems is critical for medical countermeasure research.
Product Description
Nipah Virus
This HA-NiV is a novel rapid hybrid alpha-pseudovirus assembled from the four Nipah virus structural proteins (F, G, M, N) with an alphavirus-derived reporter RNA payload. BSL-2 safe and ready to use for viral entry studies. Reporter expression in target cells within 24 hours of transduction.
Product Specifications
| Catalog Number | 0570-PP-001 |
|---|---|
| Full Name | Nipah Virus-like Particles (HA-NiV) |
| Structural Proteins | F (fusion), G (glycoprotein/attachment), M (matrix), N (nucleocapsid) |
| Reporter Payload | Alphavirus-derived self-replicating RNA encoding Firefly Luciferase, GFP, or RFP |
| Particle Type | Single-cycle alpha-pseudovirus (BSL-2 safe) |
| Target Cells | Ephrin B2/B3-expressing cells (e.g., HEK293T) |
| Form | Liquid |
| Size | 1X Concentrated: 25 × 200 µL (~100 wells per 96-well plate) |
| Storage | -80°C. Avoid repeated freeze-thaw cycles. |
Applications
- Rapid NiV pseudovirus transduction of target cells for viral entry and functional studies
- Anti-NiV drug and entry inhibitor screening
- Anti-NiV neutralizing antibody screening and IC50 determination
Safety & Handling
Live Nipah virus requires BSL-4 containment, which is unavailable to most research groups. HA-NiV is a single-cycle BSL-2-safe pseudovirus that allows viral entry studies, antibody neutralization assays, and drug screening in standard BSL-2 laboratories without risk of producing infectious Nipah virus.
NiV enters cells through ephrin B2 and B3 receptors. HEK293T cells (which naturally express low levels of ephrin B2/B3) are used in the validated assay protocol. For higher transduction efficiency, HEK293T cells overexpressing ephrin B2 or B3 can be used.
The alphavirus-derived self-replicating RNA enables reporter expression within 24 hours post-transduction for luciferase, GFP, and RFP reporters. This is faster than conventional lentiviral pseudoviruses (48–72 hours).
Need a different reporter, pack size, or bulk volume? BioHippo works directly with IBT Bioservices to support custom and large-volume requests. Contact us for a quote or to discuss your project requirements.
Looking for matching antigen proteins, antibodies, or other coronavirus/filovirus research reagents? Contact BioHippo support for recommendations.
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