| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NKD1 recombinant protein (Position: D21-K368) was used as the immunogen for the NKD1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NKD1 Antibody / Naked cuticle 1 is a anti-NKD1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: NKD1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, NKD1 antibody identifies a 471-amino-acid protein localized in the cytoplasm that binds to Dishevelled (DVL) proteins, sequestering them away from the plasma membrane and preventing downstream beta-catenin stabilization. By limiting beta-catenin nuclear translocation, NKD1 represses Wnt target gene expression, balancing cell fate decisions and tissue patterning. NKD1 expression itself is induced by Wnt signaling, creating a negative feedback loop essential for maintaining signaling homeostasis.
The NKD1 gene is located on chromosome 16q12.1 and encodes a conserved protein containing an EF-hand-like calcium-binding motif and a Dishevelled-binding region. It functions in developmental processes such as neural tube formation, axis specification, and organogenesis. In adult tissues, NKD1 continues to modulate Wnt activity to maintain epithelial integrity and stem cell renewal. Loss or silencing of NKD1 leads to excessive Wnt pathway activation, contributing to oncogenesis and abnormal cell proliferation.
In cancer research, NKD1 acts as a tumor suppressor. Reduced NKD1 expression has been observed in colorectal, gastric, and hepatocellular carcinomas, where Wnt hyperactivation drives tumor progression. Restoration of NKD1 expression inhibits beta-catenin accumulation and reduces metastatic potential, highlighting its role as a critical checkpoint in Wnt pathway regulation. Beyond oncology, NKD1 also influences bone formation, neurogenesis, and intestinal regeneration through its modulation of Wnt and planar cell polarity signaling.
NKD1 antibody is widely used in developmental biology, oncology, and cell signaling research. It is suitable for immunoblotting, immunofluorescence, and co-immunoprecipitation to investigate NKD1 expression, localization, and interaction with Wnt signaling components. Detection of NKD1 provides insight into Wnt feedback regulation and its implications in stem cell biology and cancer. In tissue models, NKD1 serves as a molecular marker for Wnt pathway inhibition and differentiation status.
Structurally, NKD1 contains conserved motifs that enable direct binding to DVL proteins and membranes enriched in phosphatidylinositol phosphates. Its function is regulated by phosphorylation and intracellular calcium levels.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
