| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human Neurokinin 1 Receptor was used as the immunogen for the NKR1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NKR1 Antibody / Neurokinin 1 Receptor / TACR1 is a anti-NKR1 Rabbit antibody Recombinant Rabbit Monoclonal clone AFBH-20 supplied in Liquid format. Recommended for workflows such as Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, cell membrane.
Key elements and design rationale
- Target: NKR1
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone AFBH-20, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): IP, IHC, WB
Biological background
Binding of Substance P to the Neurokinin 1 receptor triggers activation of phospholipase C (PLC), leading to inositol trisphosphate (IP3) production, calcium release, and activation of protein kinase C (PKC). Downstream pathways include MAPK, ERK, and NF-kB signaling cascades that regulate neuronal excitability, cytokine production, and vascular permeability. This receptor is broadly expressed in the central nervous system-particularly in pain-processing regions such as the dorsal horn of the spinal cord-and in peripheral tissues including smooth muscle, endothelial cells, and immune cells.
The NKR1 antibody is widely used in neuroscience, pharmacology, and inflammation research to study Substance P signaling, pain transmission, and GPCR-mediated pathways. Western blot analysis detects a 46 kilodalton band corresponding to the receptor, while immunohistochemistry reveals strong membrane and cytoplasmic staining in neurons and smooth muscle cells. This antibody supports investigations into tachykinin signaling and receptor modulation across different tissues.
Pharmacologically, Neurokinin 1 receptor antagonists such as aprepitant and fosaprepitant are used clinically to treat nausea, vomiting, and depression, underscoring the receptor's therapeutic relevance. Dysregulation of TACR1 signaling contributes to chronic pain, anxiety, inflammatory bowel disease, and certain cancers, where Substance P promotes cell proliferation and angiogenesis. The NKR1 antibody provides a valuable tool for characterizing receptor expression, ligand binding, and intracellular signaling dynamics under physiological and pathological conditions.
At the molecular level, Neurokinin 1 receptor undergoes desensitization and internalization following ligand binding, a process mediated by beta-arrestins and receptor phosphorylation. It can recycle back to the plasma membrane or undergo degradation depending on signal intensity. The NKR1 antibody facilitates the study of receptor trafficking, desensitization, and GPCR signaling integration.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
