| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NLE1 recombinant protein (Position: M1-R385) was used as the immunogen for the NLE1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NLE1 Antibody / Notchless protein homolog 1 is a anti-NLE1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: NLE1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Notchless protein homolog 1 contains WD-repeat motifs that facilitate interactions with nucleolar proteins involved in ribosome assembly, including NOP7 and RPF2. It promotes 60S subunit maturation by assisting in rRNA folding and ribosomal protein incorporation. The NLE1 antibody supports localization studies showing strong nucleolar enrichment, consistent with its essential role in ribosome production. Depletion of NLE1 results in defective ribosome biogenesis, p53 activation, and cell-cycle arrest.
During embryonic development, Notchless protein homolog 1 influences cell-fate determination through modulation of Notch receptor signaling. Loss of NLE1 disrupts somite segmentation and neurogenesis in animal models. The NLE1 antibody allows analysis of these regulatory interactions, helping delineate how nucleolar activity intersects with developmental gene networks. Its dual role in ribosome formation and signaling regulation underscores the integration of biosynthetic and morphogenetic processes.
In cancer research, altered ribosome biogenesis and nucleolar stress contribute to tumorigenesis. Notchless protein homolog 1 expression correlates with proliferative capacity, making it a useful marker for studies of nucleolar dynamics in malignant tissues. The NLE1 antibody supports evaluation of nucleolar size, rRNA synthesis rate, and ribosomal assembly integrity in cancer and stress-response models.
The NLE1 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing prominent nucleolar staining.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
