| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NLRP2 recombinant protein (Position: H80-D1044) was used as the immunogen for the NLRP2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NLRP2 Antibody / NALP2 is a anti-NLRP2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: NLRP2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, FACS, IP, IF, ICC, WB
Biological background
NLRP2 expression is found in oocytes, testis, brain, and immune tissues. In germ cells and early embryos, NLRP2 contributes to epigenetic regulation of imprinted gene expression and maintenance of zygotic genome integrity. Mutations in NLRP2 are associated with imprinting disorders such as Beckwith-Wiedemann and multilocus imprinting disturbances. In immune cells, NLRP2 serves as a negative regulator of inflammasome activity, limiting excessive IL-1beta secretion and preventing aberrant inflammation. Its dual role in reproduction and immunity underscores its importance in maintaining both developmental and immune homeostasis.
The NLRP2 antibody is used in immunology, developmental, and reproductive research to study inflammasome signaling and maternal effect gene function. Western blot analysis reveals a 115 kilodalton band, while immunofluorescence shows cytoplasmic and perinuclear staining consistent with inflammasome localization. The antibody helps characterize NLRP2's interactions with ASC and caspase-1 in immune signaling complexes. In reproductive studies, it assists in analyzing NLRP2's role in zygotic reprogramming and early embryonic gene activation. Loss or mutation of NLRP2 disrupts normal embryogenesis and can cause early developmental arrest.
In addition to its developmental and immunological roles, NLRP2 may contribute to cancer and neurological disease by modulating inflammation and apoptosis. Reduced expression is observed in gliomas and certain carcinomas, while overactivation of inflammasome signaling has been linked to neuroinflammation and tissue injury. The NLRP2 antibody thus supports research across multiple fields, from innate immunity to epigenetic regulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
