| Field | Specification |
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| Mfr No | |
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| Host | |
| Immunogen | A synthesized peptide derived from human NMI was used as the immunogen for the NMI antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NMI Antibody / N myc and STAT interactor is a anti-NMI Rabbit antibody Recombinant Rabbit Monoclonal clone 30N95 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP) with listed reactivity in Human.
Key elements and design rationale
- Target: NMI
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30N95, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF, IP
Biological background
NMI antibody is widely used in immunology, oncology, and signal transduction research. NMI expression is induced by interferons and other cytokines, where it modulates STAT mediated transcription. It also interacts with c-Myc and N-Myc, affecting oncogenic transcriptional programs. By detecting NMI, researchers can explore how transcriptional coactivators regulate immune signaling and cancer pathways.
In western blot assays, NMI antibody identifies protein bands of expected size in cytokine stimulated cells. Immunohistochemistry reveals expression in immune tissues, while immunofluorescence highlights nuclear and cytoplasmic distribution depending on activation state. These applications provide robust tools for analyzing NMI activity in signaling networks.
NMI has been implicated in tumorigenesis. Its expression is elevated in breast and cervical cancers, where it interacts with oncogenes and alters signaling outcomes. Conversely, NMI may also play tumor suppressive roles in certain contexts, reflecting its complex regulation. By applying NMI antibody, scientists can dissect the dual roles of this coactivator in cancer biology.
NMI also regulates antiviral responses and inflammation by modulating STAT signaling downstream of interferons. This positions NMI as a bridge between innate immunity and transcriptional responses. Its interactions with multiple transcription factors highlight its versatility in shaping gene expression outcomes.
NMI antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
