| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NMNAT1 recombinant protein (Position: K56-T279) was used as the immunogen for the NMNAT1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NMNAT1 Antibody / Nicotinamide mononucleotide adenylyltransferase 1 is a anti-NMNAT1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: NMNAT1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ICC/IF, FACS, ELISA
Biological background
Genetic studies have established that NMNAT1 is essential for embryonic viability and neuronal survival. Mutations in the NMNAT1 gene cause Leber congenital amaurosis type 9 (LCA9), a severe inherited retinal dystrophy characterized by early-onset vision loss due to impaired photoreceptor maintenance. The enzyme's role in maintaining nuclear NAD+ pools underlies its protective effects against oxidative and genotoxic stress. Beyond ocular disease, NMNAT1 contributes to genomic stability by supporting PARP-dependent DNA damage responses. Cellular NAD+ depletion impairs repair efficiency, highlighting NMNAT1 as a therapeutic target for age-related degeneration and metabolic disorders.
In neurobiology, NMNAT1 has gained attention for its neuroprotective potential. Experimental models demonstrate that increasing NMNAT1 expression can delay axonal degeneration following injury, a phenomenon known as Wallerian degeneration slow (WldS). This protective capacity likely arises from preserved nuclear NAD+ synthesis, influencing downstream survival pathways. Moreover, NMNAT1 coordinates with sirtuin-1 (SIRT1) to regulate gene expression and chromatin structure through histone deacetylation mechanisms. Disruption of this balance is linked to premature cellular aging and retinal degeneration.
The NMNAT1 antibody is a critical reagent for probing NAD+ metabolism, DNA repair, and neuroprotection pathways. Researchers use it for western blotting, immunocytochemistry, and immunohistochemistry to detect endogenous NMNAT1 across cell types. Its nuclear localization pattern provides a distinct marker for studying NAD+ biosynthesis compartmentalization. In clinical research, NMNAT1 expression is analyzed in retinal tissues, cancer cell lines, and aging models to evaluate metabolic resilience. The antibody's specificity allows discrimination between NMNAT1 and its cytoplasmic and mitochondrial isoforms, ensuring accurate interpretation of NAD+-related experiments.
Because of its central role in redox regulation, NMNAT1 represents a molecular intersection between energy metabolism, genome integrity, and cellular stress responses. By targeting this enzyme, the NMNAT1 antibody supports studies into retinal dystrophy, neurodegeneration, and therapeutic NAD+ augmentation strategies.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
