| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NNMT recombinant protein (Position: K8-L264) was used as the immunogen for the NNMT antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NNMT Antibody / Nicotinamide N-methyltransferase is a anti-NNMT Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: NNMT
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, IP, ELISA
Biological background
Nicotinamide N-methyltransferase is expressed in various tissues, with the highest levels in liver, adipose tissue, and skeletal muscle. By regulating nicotinamide levels, NNMT indirectly affects sirtuin activity and NAD+-dependent deacetylation pathways. The NNMT antibody supports research into how this enzyme modulates mitochondrial function, stress resistance, and cellular metabolism. Its detection across metabolic tissues allows mapping of expression patterns that correlate with nutrient availability and hormonal regulation.
Dysregulation of NNMT expression is associated with multiple diseases. Overexpression promotes tumorigenesis by altering cellular methyl donor availability and epigenetic modifications. Elevated Nicotinamide N-methyltransferase activity has been observed in cancers of the liver, kidney, and pancreas, where it contributes to proliferation and metastasis. Conversely, reduced NNMT activity has been linked to insulin resistance and altered lipid metabolism. The NNMT antibody is widely used to quantify enzyme levels and evaluate its role in metabolic reprogramming, energy balance, and disease progression.
In metabolic research, Nicotinamide N-methyltransferase is recognized as a master regulator of one-carbon metabolism, influencing the methionine cycle and SAM/SAH ratio. The NNMT antibody enables functional studies exploring how modulation of enzyme activity impacts methylation reactions and gene expression. Additionally, NNMT-derived metabolites such as 1-MNA exhibit signaling functions that affect endothelial function and anti-inflammatory responses, broadening the enzyme's physiological relevance.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
