| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NOL9 recombinant protein (Position: E131-N636) was used as the immunogen for the NOL9 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NOL9 Antibody / Nucleolar protein 9 is a anti-NOL9 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Nuclear.
Key elements and design rationale
- Target: NOL9
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
Nucleolar protein 9 localizes to the granular component of the nucleolus, where late steps of ribosome biogenesis occur. It interacts with the Las1L complex, which couples endonucleolytic cleavage with phosphorylation of the pre-rRNA substrate. The NOL9 antibody supports detailed localization studies, showing characteristic punctate nucleolar staining consistent with ribosome assembly regions. Depletion of NOL9 disrupts pre-rRNA maturation and impairs large ribosomal subunit formation, leading to nucleolar stress and p53 activation.
Beyond ribosome biogenesis, Nucleolar protein 9 has been linked to cell growth regulation and stress responses. It participates in pathways that maintain nucleolar integrity under conditions of transcriptional or metabolic stress. The NOL9 antibody allows researchers to assess these processes and to investigate how nucleolar function integrates with global gene-expression control. Its activity is upregulated during proliferation, reflecting the dependence of ribosome production on cellular growth signals.
Disruption or downregulation of NOL9 has been observed in cancer and developmental disorders associated with defective ribosome synthesis. The NOL9 antibody provides a tool for analyzing expression patterns in tumor and normal tissues, supporting exploration of ribosome biogenesis as a therapeutic target. Elevated NOL9 expression correlates with high ribosomal demand in rapidly dividing cells, making it a marker of proliferative capacity.
The NOL9 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing strong nucleolar localization.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
