| Field | Specification |
|---|---|
| Mfr No | |
| Product Format | Frozen |
| Product Type | |
| Shipping | |
| Storage |
Overview
Kupffer Cells, also known as Browicz-Kupffer cells and stellate macrophages, are specialized macrophages located in the liver lining the walls of the sinusoids. Upon activation Kupffer cells release various products like cytokines, prostanoides, nitric oxide and reactive oxygen species. The product is supplied as frozen cells.
Key elements and design rationale
- Biological source: Human (H. sapiens)
- Tissue origin: Liver
- Growth properties: Adherent, polygonal
- Format: Frozen
- Seeding guidance: 13,000 - 15,000 cells/cm²
- Biosafety level: II
- Culture context: PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels.
Monocyte- and macrophage-lineage cells coordinate innate immune sensing, cytokine production, phagocytosis, and tissue-specific inflammatory responses.
Research relevance and current trends
- Primary innate immune cell models are widely used to assess activation-state plasticity and cytokine output.
- Researchers often study tissue-specific macrophage phenotypes or donor-dependent inflammatory responses.
- Co-culture systems help reveal crosstalk with stromal, epithelial, endothelial, or parenchymal cells.
Common research applications
- Measure inflammatory mediator release and activation markers after stimulation.
- Use in phagocytosis-focused or co-culture assays requiring primary innate immune cells.
- Evaluate tissue-specific immune crosstalk in organ-relevant model systems.
Product-specific data supplied for this listing
- Growth Conditions: PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM5549) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Note: Cells require additional time to recover from post-thaw conditions. Change media every day after the first 48 hours post-thaw, or earlier if media starts to change colour to yellow.
- Seeding Density (cells/cm²): 13,000 - 15,000
Notes for experimental interpretation
- Primary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.
- Attachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.
- Cryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 100xg for 7 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a pre-coated T75 culture flask.
- Incubate the cells at the recommended conditions.
- Allow cells to recover undisturbed during the first 48 hours post-thaw.
- Change media every 24 hours thereafter, or earlier if a media colour change is observed.
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
