| Field | Specification |
|---|---|
| Mfr No | |
| Product Format | Frozen |
| Product Type | |
| Shipping | |
| Storage |
Overview
Normal Human Primary Hepatic Stellate Cells are human frozen primary cells from liver used for liver fibrosis, extracellular matrix remodeling, and hepatic stromal signaling studies. These cells display adherent, polygonal growth characteristics.
Key elements and design rationale
- Biological source: Human (H. sapiens)
- Tissue origin: Liver
- Growth properties: Adherent, polygonal
- Format: Frozen
- Seeding guidance: 4,000 cells/cm²
- Biosafety level: II
- Culture context: PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels.
Hepatic stellate cells regulate extracellular matrix turnover and are widely studied in liver injury and fibrosis models because of their ability to transition toward activated, matrix-producing states.
Research relevance and current trends
- Primary stellate cells are used to examine activation-state transitions and fibrosis-associated matrix programs.
- Co-culture systems with hepatocytes, endothelial cells, or immune cells help model multicellular liver injury responses.
- Researchers frequently compare quiescent and activated phenotypes under defined cytokine or matrix conditions.
Common research applications
- Assess activation markers, matrix production, and profibrotic signaling under defined stimuli.
- Model hepatic stromal crosstalk in co-culture or conditioned-media experiments.
- Use in fibrosis-focused pathway studies and screening assays.
Product-specific data supplied for this listing
- Growth Conditions: PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. Sinusoidal Endothelial Cell Growth Medium (TM086) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Note: for best results, allow cells to recover during the first 12 hours post-thaw. Change media 24 hours after thawing cells and every other day, thereafter.
- Warranty: abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period”.
- Disclaimer: 1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at orders@biohippo.com.
- Seeding Density (cells/cm²): 4,000
Notes for experimental interpretation
- Primary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.
- Attachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.
- Cryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).
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🧊 Thawing Protocol
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.
- Incubate the cells at the recommended conditions. Do not disturb cells for at least 12 hours after seeding.
🔬 Subculture Protocol
Volumes given below are for a T75 flask; proportionally increase or decrease the volume as required per culture vessel size. Subculture cells once the culture vessel is 90% confluent.
- Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel.
- Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37°C, for several minutes to facilitate detachment.
- Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel.
- Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.
- Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired.
- Incubate the cells at the recommended conditions.
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
Do I need Applied Cell Extracellular Matrix (G422) if I am using PriCoat™ flasks?
Please refer to the Growth Conditions section of your specific product page. This section will indicate whether Applied Cell Extracellular Matrix (G422), PriCoat™ flasks, or both are recommended for optimal cell attachment and growth, as requirements may vary by cell type.
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