| Field | Specification |
|---|---|
| Mfr No | |
| Product Format | Frozen |
| Product Type | |
| Shipping | |
| Storage |
Overview
Normal Human Small Airway Epithelial Cells are human frozen primary cells from airway used for barrier biology, transport, host-pathogen interactions, and tissue-specific epithelial response studies. These cells display adherent, epithelial growth characteristics.
Key elements and design rationale
- Biological source: Human (H. sapiens)
- Tissue origin: Airway
- Growth properties: Adherent, epithelial
- Format: Frozen
- Seeding guidance: 6,666 cells/cm²
- Biosafety level: II
- Culture context: Use of PriCoat™ T25 Flasks (G299) coated with Human Type IV Placental Collagen is recommended for optimal cell attachment and propagation.
Epithelial cells form polarized barriers that regulate transport, secretion, and defense at tissue interfaces. Their phenotype is shaped by cell polarity, junctional organization, and tissue-specific differentiation cues.
Research relevance and current trends
- Primary epithelial models are widely used to examine barrier integrity, cytokine responses, and transport processes under physiologic conditions.
- Researchers often combine epithelial cells with stromal or immune components to model tissue microenvironments more faithfully.
- Donor-matched or disease-context epithelial cells help capture inter-individual heterogeneity in response assays.
Common research applications
- Measure barrier integrity, junctional organization, and polarized responses in vitro.
- Evaluate cytokine, pathogen, or drug responses in tissue-relevant epithelial cultures.
- Use in co-culture or air-liquid interface-adjacent workflows when epithelial context matters.
Product-specific data supplied for this listing
- Growth Conditions: Use of PriCoat™ T25 Flasks (G299) coated with Human Type IV Placental Collagen is recommended for optimal cell attachment and propagation. Wash with 1X PBS (Mg/Ca) twice times, at least, prior to plating cells. Bronchial Endothelial Cell Growth Medium (TM092) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Note: warm media to room temperature and protect from light. Media freshness is vital for cell health and propagation.
- Warranty: abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period”.
- Population Doubling Time (h): 35-45
- Seeding Density (cells/cm²): 6,666
Notes for experimental interpretation
- Primary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.
- Attachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.
- Cryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).
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🧊 Thawing Protocol
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- DO NOT CENTRIFUGE FRESHLY THAWED CELLS. Transfer the vial contents to a 6 well plate with 2 ml fresh, warm complete growth media.
- Incubate the cells at the recommended conditions.
- Break up cell clumps through gentle pipetting, the next day. Replace media with fresh media, every other day.
🔬 Subculture Protocol
Once cells become 75% confluent, detach with Accutase and expand to larger culture vessel, as appropriate. Do not seed cells at very low densities.Volumes given below are for a T75 flask; proportionally increase or decrease the volume as required per culture vessel size. Subculture cells once the culture vessel is 75% confluent.
- Aspirate the culture media, wash with 5ml 1X PBS without magnesium and calcium. Incubate with 10 ml 1X PBS supplemented with 1 mM EDTA for 3-7 minutes at 37°C.
- Add 5 ml of fresh room temperature Accutase to the culture vessel and let sit at room temperatire for 10 minutes. Periodically observe cells for rounding, using a microscope.
- Detach cells by gently tapping the flask, once the cells appear well-rounded. Collect the cell culture suspension and transfer to 50 ml conical tube without a filter. Pipette the cell solution several times to break up any clumps.
- Apply the cell solution to a filter to strain out clumps' single cell suspension is helpful to achieving a monolayer. Wash cells with 5ml media, and centrifuge at 300xg for 10 minutes.
- Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired.
- Incubate the cells at the recommended conditions.
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
Do I need Applied Cell Extracellular Matrix (G422) if I am using PriCoat™ flasks?
Please refer to the Growth Conditions section of your specific product page. This section will indicate whether Applied Cell Extracellular Matrix (G422), PriCoat™ flasks, or both are recommended for optimal cell attachment and growth, as requirements may vary by cell type.
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