| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NOXO1 recombinant protein (Position: M1-E294) was used as the immunogen for the NOXO1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NOXO1 Antibody / NADPH oxidase organizer 1 is a anti-NOXO1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: NOXO1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ELISA
Biological background
Functionally, NOXO1 antibody identifies a 376-amino-acid protein containing PX (phox homology) and SH3 (Src homology 3) domains that mediate membrane localization and protein-protein interactions. NOXO1 binds to membrane subunits such as NOX1, NOXA1, and p22phox, organizing them into an active complex that transfers electrons from NADPH to oxygen, generating superoxide. This process supports cellular signaling, host defense, and redox homeostasis.
The NOXO1 gene is located on chromosome 16q22.1 and encodes a regulatory component expressed in epithelial cells of the colon, prostate, and vascular tissues. Unlike its phagocytic counterpart p47phox, NOXO1 lacks an autoinhibitory region, resulting in constitutive activity under resting conditions. Its expression enables sustained ROS generation in nonimmune cells for physiological signaling functions such as cell proliferation and differentiation.
In redox signaling, NOXO1-derived ROS modulate kinase activation, ion transport, and gene expression. In the intestine, NOXO1 contributes to mucosal defense and microbial balance by supporting NOX1-mediated oxidative activity. In vascular and neural tissues, it influences smooth muscle tone and neuronal excitability. Dysregulation of NOXO1 expression or complex assembly has been implicated in oxidative stress-related disorders, including inflammatory bowel disease and hypertension.
NOXO1 antibody is widely used in redox biology, signaling, and oxidative stress research. It is suitable for western blotting, immunofluorescence, and co-immunoprecipitation to analyze NOXO1 expression and complex formation. This antibody aids in studying NADPH oxidase regulation, reactive oxygen generation, and oxidative signaling pathways. It is also useful in exploring NOX1 and NOX3 complex activity in epithelial and neuronal systems.
Structurally, NOXO1 features an N-terminal PX domain that binds phosphoinositides and C-terminal SH3 motifs that engage proline-rich regions of target proteins. Post-translational regulation includes phosphorylation and redox-dependent modifications that modulate its interaction with NOXA1 and NOX1.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
