| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NPRL2 recombinant protein (Position: D90-K380) was used as the immunogen for the NPRL2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NPRL2 Antibody / Nitrogen permease regulator-like 2 is a anti-NPRL2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Mouse, Rat.
Key elements and design rationale
- Target: NPRL2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
NPRL2 is encoded by the NPRL2 gene located on human chromosome 3p21.3, a region frequently deleted in several cancers. The protein is approximately 380 amino acids long and forms a complex with NPRL3 and DEPDC5 to constitute the GATOR1 inhibitory module. NPRL2 localizes to both cytoplasm and lysosomes, where it acts upstream of Rag GTPases to suppress mTORC1 activity under nutrient-limiting conditions.
The NPRL2 antibody detects a 45 kilodalton band by western blot and reveals punctate cytoplasmic staining consistent with lysosomal localization. NPRL2 is required for proper cellular adaptation to nutrient stress, promoting autophagy and metabolic reprogramming. Loss of NPRL2 results in constitutive mTORC1 activation, uncontrolled cell proliferation, and impaired stress response.
As a tumor suppressor, NPRL2 modulates DNA damage repair and enhances sensitivity to chemotherapeutic agents such as cisplatin. Reduced NPRL2 expression has been documented in lung, colorectal, and breast cancers, correlating with poor prognosis and therapy resistance. Restoring NPRL2 expression reactivates nutrient stress signaling and suppresses tumor growth through mTOR inhibition and apoptosis induction.
Through its dual role in nutrient sensing and cancer suppression, NPRL2 serves as a key regulator of cellular homeostasis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
