| Field | Specification |
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| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NPTX2 recombinant protein (Position: G16-L431) was used as the immunogen for the NPTX2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NPTX2 Antibody / Neuronal pentraxin 2 is a anti-NPTX2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: NPTX2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ELISA
Biological background
NPTX2 is encoded by the NPTX2 gene located on human chromosome 7q21.3. The protein is approximately 47 kilodaltons and secreted from presynaptic terminals, where it binds AMPA-type glutamate receptors on postsynaptic neurons. This interaction enhances receptor clustering, synaptic strength, and excitatory transmission. NPTX2 is highly expressed in cortical and hippocampal neurons and plays a key role in activity-dependent synaptic refinement during learning and memory.
The NPTX2 antibody detects a 47 kilodalton band on western blot. In the brain, NPTX2 is upregulated by neuronal activity and acts in concert with NPTX1 and NPTR to regulate synaptic homeostasis. Disruption of NPTX2 expression leads to impaired long-term potentiation and deficits in learning and behavior. Cerebrospinal fluid levels of NPTX2 have been proposed as a biomarker for early-stage neurodegeneration, with reductions correlating to cognitive decline in Alzheimer's and frontotemporal dementia.
At the molecular level, NPTX2 binds to neuronal pentraxin receptor (NPTXR) to form hetero-oligomeric complexes that tether AMPA receptors to the postsynaptic membrane. This complex promotes excitatory synapse stabilization and neurotransmitter signaling efficiency. Dysregulation of this system contributes to excitatory-inhibitory imbalance, a hallmark of several neuropsychiatric and neurodegenerative conditions.
Beyond the nervous system, NPTX2 expression has been observed in pancreatic islets and endocrine tissues, where it may regulate calcium-dependent secretion.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
